Cultivation

PC bottle with leakproof PP screw cap. With moulded graduations and pouring rim. Ideal for storing aqueous solutions and for the short-term storage of cell culture media. Autoclavable.

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Improved cell adhesion through physical surface treatment
• Cell culture flasks with filter screw cap
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 25 cm², 75 cm² and 175 cm² growth areas
• High and flat design of the 175 cm² cell culture flask for variability of media volume

These standard surface treated cell culture plates for the cultivation of adherent cells exhibit a reduced evaporation due to raised well rims and uniform rings on the lids.

• Available with 4-, 6-, 12-, 24-, 48-, 96- or 384-wells (lid included)
• Flat well bottom
• 96-well plate also available as round bottom plate (6.267 171)
• Single position lid reduces the risks of cross-contaminations
• Wells are labeled with alphanumeric code for easy identification
• Sterilized by gamma irradiation
• Pyrogen-free
• Material: Polystyrene

4- to 48-well multidishes with Nunclon™Δ treated surface. 6-well plates also available with Poly-D-Lysine and Collagen I coating.
Raised well rims lower the risk of cross contamination.
Excellent optical quality. All available surface modifications are certified pyrogen-free and tested for both monolayer
formation and cloning efficiency employing primary cells as well as repeating cell lines. Sterile. With lid
Material dishes and lids: Polystyrene

Nunclon™Δ cell culture flasks with surface areas from 25 to 175cm² are available with filter caps or Vent/Close caps for good gas exchange. Short, wide neck (angled or straight) allows easy access to entire growth surface. The Nunclon™Δ treatment is limited to the intended growth area. Neck areas are not treated to prevent cell attachment and growth in undesired areas. Certified pyrogen-free and tested for both monolayer formation and cloning efficiency employing primary cells as well as repeating cell lines. Sterile.
Material flasks: Polystyrene
Material caps: HDPE

With white HDPE screw closure. Reduced permeability to CO₂/O₂ which reduces pH-shift. Bottles and closures are radiation-sterilised and pyrogen-free to eliminate costly washing, depyrogenation and autoclaving steps. Heatshrink band around closure and neck provides tamper-evident seal. Packed in shrink-wrapped trays. 2L size has molded-in handgrips and a 53-mm (53B) white closure. Bottles are sterile to 10-6 SAL, non-pyrogenic, non-cytotoxic and comply with USP Class VI guidelines. Sterile. Transparent. Leakproof. Graduated.

ThinCert™ Cell Culture Inserts for advanced cell and tissue culture applications. They are compatible with standard CELLSTAR® multiwell plates. The coupling between the housing and the membrane is achieved using an automated process which produces an extremely strong and robust seal without compromising or weakening the membrane in any way. The membranes undergo a physical surface treatment to optimise cellular adherence and growth characteristics. Due to a high chemical resistance to solvents a broad spectrum of cell fixation protocols can be performed. The specific hanging geometry design of the ThinCertTM cell culture inserts ensures that there is always a gap between the membrane support and the bottom of the well.

Proporties:

• Stable clear polystyrene housing, USP class VI certified and cell culture compatible
• Hanging geometry
• Sealed PET capillary pore membrane
• Single, sterile blister packing
• Improved cell adhesion through physical surface treatment
• Simplified pipetting due to self-lift geometry
• Enhanced pipetting access and gas exchange

In the case of concentration determinations in microplates, the measurement is made vertically and the layer thickness of the sample to be measured is dependent on the sample volume. Even with smaller sample volumes, the resulting layer thicknesses are sufficient for precise measurement. At a constant sample volume, concentrations can be determined with the aid of a calibration curve.

Properties:

• Optical window down to 230 nm ideal for nucleic acid determinations at 260 nm/280 nm
• For measurements of protein concentration at 280 nm
• Transmission measurements with UV light up to 230 nm
• DMSO-resistant
• Stored at -20 °C without any problem

Minimize plate evaporation through the uniquely engineered design of Thermo Scientific™ Nunc™ Edge-96 Well Plate. The edge plate is engineered for optical superiority allowing your automated imaging systems to operate efficiently. The plates are made of clear polystyrene for excellent optical properties making it ideal for optical measurements
Features:

• Reduced Edge Effect virtually eliminates evaporation in lengthy incubations
• Overall plate evaporation rate reduced to <2% after seven days of incubation
• Low evaporation permits full-plate results
• Large evaporative buffer zones eliminate well-to-well variability
• Perimeter moat can be filled with 1.7mL sterile H2O or media
• Low background fluorescence, ideal for optical instruments
• Ideal for image-based high content applications like measuring primary neuron cultures
• Customized bar coding available (upon request)
• Untreated or cell culture treated hydrophilic surface (Nunclon™ Δ)