Cultivation

Clear, white or black plates with excellent optical clarity and flat bottom wells. Nunclon™Δ, MaxiSorp™, PolySorp™, Collagen I, Poly-D-Lysine surface modifications suitable for cell culture assays.
Material plates: Polystyrene
Total volume µl/well: 400

Optimizing spheroid formation

• Allows cells to grow in suspension with virtually no cell attachment
• Supports many different cell types and their ability to generate spheroids
• Enables consistent, repeatable cell growth

• Minimizes variability and supports the consistent formation of cancer spheroids that simulate the 3D structures of tumor growth, providing a better model system for studying tumor cell progression and efficacy of anticancer agents in vitro

• Demonstrates superior quality for embryoid body formation of pluripotent stem cells with minimal spontaneous differentiation
• Resultant cells are able to differentiate into all three germ layers

• Free of animal products
• Pyrogen-free: Endotoxin tested (0.5 EU/mL)
• Biologically inert: USP biological reactivity class VI
• High level of sterility: SAL 10-6, ethylene oxide gas sterilization
• Shelf Life 4 years

With TPE gasket and port caps, Nalgene 50 platinum-cured silicone tubing. All plastic screw closures for sterile liquid transfer of tissue media, biological reagents, pure water and chemicals to and from Nalgene-carboys. For applications such as bio-reactors and fermenters. Suitable for all large Nalgene carboys or bottles with screw closures of 53mm (53B) or 83mm (83B). Includes two Nalgene 550 platinum-cured silicone tubing for drop tube and splash guard. Can be used with Nalgene carboy bottles with properly set up peristaltic pumps. Autoclavable.

Cell culture flasks with Nunclon™Δ. Surface modifications are certified pyrogen-free and tested for monolayer formation and cloning efficiency with primary and immortalized cell lines. Sterile. Bottleneck at an angle. Sterile.
Material flasks: Polystyrene
Material caps: HDPE

These cell culture flasks with treated surfaces for the cultivation of adherent cells are available with different growth areas from 12.5 to 300 cm². Available with vented or plug-seal caps made from polyethylene. Vented caps contain a 0.22 µm hydrophobic filter to allow gas exchange and minimize risk of cross-contamination. Plug-seal caps can be used in closed systems, providing a liquid and gas sheer seal, but by simply unscrewing the cap one quarter of a turn, this cap can also be used in an open system.

• Innovative angled neck design offers good pipette and cell scraper access
• Upper triangular and wider base shape provides stability
• Stackable
• Special writing area near the neck
• Engraved graduation on both flask sides
• Sterilized by gamma irradiation
• Pyrogen-free
• Individually packed in resealable outer packaging with zip fastener

With blue vented closure, the chance for cross contamination is reduced. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG. Molded-in graduations. Leak-proof HDPE closure has a hydrophobic 0.2µm PTFE membrane that allows sterile air exchange. Flasks offer a 5-year shelf life, a 10^-6 SAL, are non-pyrogenic and non-cytotoxic. Available with flat or baffled bottom. Individually packaged for easy storage and handling.

Chamber slides eliminate the need for cell transfer prior to visualisation / staining. Upper structure can be removed when culturing is complete. Useful for viral and mycoplasma testing, chromosome studies, toxicity tests and immunocytology. Sterile. The chemically coated growth surface of the Lab-Tek™II-CC² Chamber Slide System mimics polylysine and provides binding sites optimal for fastidious cells (e.g. neurons).
Material chamber: Polystyrene

Lab-Tek™: Sealant: Non-toxic medical grade silicone.
Lab-Tek II™: Sealant, biocompatible acrylic adhesive.
Lab-Tek II - CC²: modified glass slide, chemically coated to mimic polylysine.

The optimised design of the scraper blade features a pivot angle of 60° that facilitates uniform contact with the growth surface, minimal mechanical strain and efficient cell harvest, even from poorly accessible surfaces. The scraper design also minimises any
accumulation of cell suspension to the blade structure. The handle length has been adapted for use.

Proporties:

• For gentle mechanical removal of adherent cells
• Optimised blade design for maximum cell harvest
• Blade length: 1.8 cm
• Minimal mechanical strain
• 28 cm and 40 cm handhold length
• Pivot angle 60°
• Sterile individual packaging

Available in two different lengths with adjustable blade for optimal application flexibility. Pyrogen-free. Sterile.

Excellent for confocal image analysis and optimal for high power inverted microscopic viewing. Sterile.
Material chamber: Polystyrene
Material slide: Glass

Lab-Tek™: connection between the chamber (s) and slides from non-toxic, medical silicone.
Lab-Tek™ II: connection between the chamber (s) and slides of biocompatible acrylic adhesive.
Medium chamber is not removable.