Cell culture cultivation

Polystyrene is particularly suitable because of the high transparency of the material for optical measurements. It is guaranteed to be sterile and non-cytotoxic and free of detectable DNase, RNase, human DNA and endotoxins.

Properties:

• Made of high-grade polystyrene
• Improved cell adhesion through physical surface treatment
• Available with screw cap, bayonet cap or two-position vent stopper

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Hydrophobic surface, ideal for suspension cultures, hybridoma and embryonic stem cells
• Suspension culture flasks with standard screw cap (without filter)
• White standard screw caps with ventilation position
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 50 ml, 250 ml, 550 ml and 650 ml volumes available

The CELLCOAT® product line comprises cell culture vessels which are coated with proteins of the extracellular matrix or synthetic proteins. Beside an improved adhesion and proliferation of primary cells and various cell lines. CELLCOAT® plates are highly suitable for serum-free and serum-reduced cell cultivation. Experiments which include additional washing steps or stressful procedures, e.g. transfection. Moreover, the differentiation of individual cell types can be enhanced through the protein-coating.
Collagen Type I is a protein of the extracellular matrix, an intercellular substance which in vivo influences adhesion, migration and proliferation among other processes. Collagen Type I represents one of the most important ECM proteins for in-vitro cell cultures.

Proporties:

• difficult-to-cultivate cells adhere show a positive growth behaviour
• positive influence on differentiation and morphology
• Cell culture flasks with filter caps
• Shelf life: 24 months at room temperature
• Further cell culture vessels coated with Collagen Type I available on request

Sterile disposable flasks with white HDPE closure reduce the chance for cross contamination. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG plastic. Molded-in graduations. Leak-proof HDPE screw closures open to vent with 1/4 turn. Flasks offer a 5-year shelf life, a 10-6 SAL, are non-pyrogenic and non-cytotoxic. Individually packaged for easy storage and handling.

Sterile disposable flasks with white HDPE closure reduce the chance for cross contamination. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG plastic. Molded-in graduations. Leak-proof HDPE screw closures open to vent with 1/4 turn. Flasks offer a 5-year shelf life, a 10-6 SAL, are non-pyrogenic and non-cytotoxic. Individually packaged for easy storage and handling.

With hydrophobic 0.2 µm PTFE membrane.

With blue vented closure, the chance for cross contamination is reduced. Ideal for shaker and suspension cell culture, media preparation or storage. Made of light, crystal clear PETG. Molded-in graduations. Leak-proof HDPE closure has a hydrophobic 0.2µm PTFE membrane that allows sterile air exchange. Flasks offer a 5-year shelf life, a 10^-6 SAL, are non-pyrogenic and non-cytotoxic. Individually packaged for easy storage and handling.

It is now easy to create a Thermo Scientific Nunc EasyFill™ Cell Factory system for your applications.

• Connections made easy: Plug a media bag or tubing set to any cell factory.
• Save time and reduce costs: Minimise cleaning processes and sterility validations. Accessories are gamma irradiated and individually wrapped.
• Prevent build up of back pressure during filling application: New venting options provide additional air venting when filling and harvesting by gravity or with a peristaltic pump.

The Nunc™ High Density Cell Factory™ System offers 30% more surface area and yield* than the standard Cell Factory system or similar multi-tray systems for adherent cell culture. Its economic benefits are immediate with impacts across many functions of an organization, as it enables end users to optimize their cell culture manufacturing footprint, reduce material usage and labor-consumption as well as to increase their cell culture yield.

With PE screw cap with silicone seal. Ready to use. With printed graduations in ml. With ribbed hand-grips (except 125 ml and 5000 ml). Materials meet current USP VI, are non-cytotoxic and pyrogen-free.