Protein blotting

An all-in-one blocking solution for Western blot analysis. In one step, blocking, primary and secondary antibodies hybridization as well as enhancing the signal.

• Enhance antibody signal
• Universal antibody diluent
• Effective with any ECL substrates
• Compatible with PVDF and NC membrane
• Improve protein detection

Extremely pure, very absorbent, smooth paper for use in all protein and nucleic acid blotting techniques.

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membrane their solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon P membrane is particularly suitable for proteins with a molecular weight greater than 20 kDa.

Features & Advantages

• 0.45 µm Pore size
• Suitable for Western Blotting
• Millipore Rapid Immunodetection Protocol reduces detection time by up to 2 hours

In contrast to nitrocellulose, Immobilon membranes have a number of advantages. An optimized coloring capacity, a broad solvent compatibility and a lower background make the membranes your solution. Immobilon membranes do not rupture or curl and can be cut without breaking. Reprobing is also possible many times. The Immobilon PSQ membranes are particularly suitable for blotting proteins with a molecular weight below 20 kDa. They also offer higher protein adsorption and therefore better results in protein sequencing than with other membranes.

Features & Benefits

• 0.2 µm pore size, textured surface
• Prevent the migration of proteins with lower molecular weight through the membrane

The omniBlot is an stand-alone unit for the tank-blot method. Each unit consists of an OmniPAGE tank and lid, a electroblotting insert as well as cassettes and fibre pads. The versatile design of the tanks and the matching lids are compatible with the vertical PAGE or IEF systems with the appropriate optional inserts.

• Mini system transfers up to 4 Mini gels
• Mini Wide system transfers up to 4 Mini Wide and 8 Mini gels
• Maxi system transfers up to 4 Maxi gels, 8 Mini Wide and 16 Mini gels
• Run-times ranging between 2 and 20 hours
• Rigid cassettes eliminate the formation of shadow bands
• Colour coded cassettes prevent reverse polarity for correct orientation
• Cooling with cooling packs at high transmission intensity

For staining blotted proteins on nitrocellulose and PVDF membranes. Colour detection for immunoblots (Western blots). Can also be used for staining proteins on cellulose acetate membranes.

• Membrane staining
• Reversible colouring
• Reusable

Webbing reinforced nitrocellulose.
High mechanical stability for multiple hybridisations.

Pure Nitrocellulose transfer membrane for all protein or immunoblotting applications. The high sensitivity and low background ensures excellent results in all transfers. Compatible with all detection systems. BSA binding capacity up to 100µg/cm².

Signal word:
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Hydrophobic Polyvinylidene Fluoride (PVDF) transfer membrane with high binding capacity and low background. Ideal for use in protein binding applications such as Western blots, solid phase assays and immunoblotting procedures. BSA binding capacity up to 125 µg/cm²