Zellkulturmedien und Reagenzien

Zellkulturmedien wurden speziell für die Kultivierung tierischer oder pflanzlicher Zellen entwickelt. Diese und viele weitere Artikel für die Zellkultur, wie Reagenzien, Antibiotika, Puffer für die Zellultur finden Sie von nahmhaften Herstellern wie Biowest und Serana bei Th. Geyer. Viele Artikel sind direkt ab Lager lieferbar.
Beachten Sie in einem separaten Bereich zusätzlich noch Seren für die Zellkultur.

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Dimethylsulfoxid, ACS, für die Zellkultur geeignet (min. 99,9 %)

BIOSOLUTE®

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2308-100ML

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 DULBECCO'S MODIFIED EAGLE MEDIUM - DMEM

BioWest

Das DMEM-Medium ist eine Modifikation des BME, welch eine höhere Konzentration an Aminosäuren, Vitaminen und anderen Zusatzstoffen enthält. DMEM dient zur Unterstützung und Aufrechterhaltung einer breiten Palette von Säugetier Zelltypen. Es gibt zwei Arten von DMEM: eins mit einem hohen Glukosegehalt (4,5 g/L) und das andere mit einem niedrigen Glukosegehalt (1,0 g/L). Das DMEM mit niedrigem Glukosegehalt wurde für die Kultur von Maus-Embryonalzellen entwickelt....

 RPMI 1640 MEDIUM

BioWest

RPMI ist ein angereichertes Allzweckmedium mit umfangreichen Anwendungsmöglichkeiten für ein breites Spektrum von Säugetier- und Hybridomzellen, einschließlich menschlicher Myelome, Maushybridome, menschlicher Leukozyten sowie B- und T-Lymphozyten. Es wurde ursprünglich für Suspensionskulturen und Monolayerkulturen von menschlichen Leukämiezellen entwickelt....

 MEM MIT HANKS' SALZEN

BioWest

Minimum Essential Medium (MEM) ist eine Abwandlung von Eagle's früherem Medium Basal Medium Eagle (BME). Diese MEM w/ Hanks' Salts Formulierung enthält Hanks' Salze zur Verwendung ohne CO2....

 MEM MIT EARLE'S SALZEN

BioWest

Minimum Essential Medium (MEM) mit Earle's Balanced Salts ist eine Modifikation von Eagle's Basalmediums (BME), das eine höhere Konzentration an essenziellen Nährstoffen enthält. Diese Medien fördern das Wachstum einer Vielzahl von normalen und transformierten Zellen. Da sie Earle's Balanced Salts enthalten, sind sie für die Verwendung in Atmosphären geeignet, die mit CO2-Gas angereichert sind....

 DMEM - HAM'S F12

BioWest

Das DMEM Ham's F12 bietet hervorragende Leistungen für bestimmte epitheliale, endotheliale und granulöse Zelltypen. Bei richtiger Supplementierung ist es ein sehr erfolgreiches Basismedium für die serumfreie Zellkultur....

 Antibiotika

BioWest

Schützen Sie Ihre Zelllinien vor Kontamination durch fremde Organismen wie Bakterien, Pilze und Viren und/oder verwenden Sie die Antibiotika zur Selektion nach der Transfektion.
Die meisten Antibiotika unterdrücken das Wachstum von Mikroorganismen, indem sie einen anabolen Stoffwechselweg blockieren....

 MEDIUM 199

BioWest

Dieses komplexe Medium wurde speziell für die Ernährungsforschung an Hühnerfibroblasten entwickelt. Heute wird das Medium 199 in großem Umfang für die Erhaltung von nicht transformierten Zellen, Impfstoff- und Virusproduktion sowie für Primärexplantate von Epithelzellen verwendet.
Die Medien können entweder mit Earle-Salzen oder Hanks-Salzen formuliert werden. Die mit den Earle-Salzen formulierten Medien sind mit einer Bikarbonat/Kohlensäure-Lösung gepuffert und behalten ihren...

 HAM'S F12 / F-12 NÄHRMEDIUM

BioWest

Ham's F12 wurde ursprünglich entwickelt für das serumfreie klonale Wachstum von Chinese Hamster Ovarzellen (CHO), Lungenzellen und Maus-L-Zellen. Es ist das Medium der Wahl für die Unterstützung von Zellen aus Nagetieren (insbesondere Kaninchen und Ratte) und hat sich als hervorragendes Klonierungsmedium für die Kultur von Myelom- und Hybridzellen (Hybridomen) bewährt....

 ISCOVE'S MODIFIZIERTES DULBECCO-MEDIUM - IMDM

BioWest

Iscoves-Medien sind angereicherte Modifikationen von DMEM mit Natriumselenit. Sie eignen sich hervorragend für schnell proliferierende Zellkulturen mit hoher Dichte. Mit dem Zusatz von BSA, gereinigtem humanem Transfer und Sojalecithin wird eine serumfreie Umgebung geschaffen, die ideal für die Unterstützung von B- und T-Lymphozyten ist. IMDM war das erste Medium, das HEPES-Puffer verwendete. Andere Zelltypen können können mit diesem Medium unter serumfreien oder serumreduzierten Bedingungen kultiviert werden....

 Basal medium eagle - BME

BioWest

BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten....

 MC COY'S 5A MEDIUM

BioWest

Mc Coy's-Medien wurden ursprünglich für das Wachstum und die Unterstützung von Lymphozyten formuliert. Aus dieser letzten Modifikation entstand ein Medium, das mit RPMI 1629 identisch ist. McCoy's 5A-Medien unterstützen die unbegrenzte Vermehrung von Walker-256-Karzinomzellen. Darüber hinaus eignet es sich hervorragend für die Vermehrung von Leukozyten, Biopsiegeweben, einer breiten Palette normaler oder transformierter Zelltypen von Mensch und Ratte, den gängigsten primären und kontinuierlichen Zelllinien....

 CMRL 1066

BioWest

CMRL-Medien sind besonders für das klonen von Affen Nierenzellen und für das Wachstum vieler anderer Säugetierzelllinien geeignet, wenn sie mit Pferde- oder Kälberserum ergänzt werden....

 GLASGOW MEM BHK 21

BioWest

Glascow Minimum Essential Medium wurde ursprünglich von Ian MacPherson und Michael Stoker als eine Modifikation von Eagle's Medium (BME) entwickelt. Die Modifikationen umfassten die Zugabe von 10 % Tryptosephosphat und die doppelte Konzentration an Aminosäuren und Vitaminen. Dieses Medium wurde verwendet, um die genetischen Faktoren zu untersuchen, die die Zellkompetenz beeinflussen....

 HAM'S F10 / F-10 NÄHRMEDIUM

BioWest

Ham's F10 ist ein beliebtes Medium für das Wachstum von anspruchsvollen Zelllinien....

 HAM'S F14 / F-14 NÄHRMEDIUM

BioWest

Ham's F14 Medium wurde aus Ham's F12 entwickelt, das ursprünglich für das
serumfreie, klonale Wachstum von Chinese Hamster Ovary (CHO)-Zellen, Lungenzellen und Maus-L-Zellen entwickelt wurde.
Ham's F14 enthält eine doppelte Konzentration an Aminosäuren im Vergleich zu Ham's F12. Dieses Produkt ist außerdem mit Calciumchlorid und Ascorbinsäure angereichert....

 LEIBOVITZ L-15 MEDIUM

BioWest

Die Leibovitz L-15-Medien wurden entwickelt, um das Zellwachstum in
Medien zu unterstützen, die kein CO2-Gleichgewicht aufweisen. Die Formulierungen wurden mit dem Natrium Bicarbonat-Puffer entwickelt. Die Leibovitz L-15-Medien sind durch eine Reihe von Salzen, freien basischen Aminosäuren und Galaktose gepuffert, so dass sie unter Bedingungen des freien Gasaustauschs mit der Atmosphäre verwendet werden können. Wenn es richtig ergänzt wird, unterstützt L-15 Medium etablierte Zelllinien, wie HEp-2 und LLC-MK2, sowie primäre Explantate von embryonalen und erwachsenen Menschen....

 SERUM REDUZIERTE MEDIEN - MCDB

BioWest

Diese Medien wurden als serumreduziertes Medium für die Kultivierung
von menschlichen mikrovaskulären Endothelzellen entwickelt. Für andere mikrovaskuläre Zellen wird es empfohlen mit Wachstumsfaktoren wie EGF oder Hormonen wie Hydorcortison zu verwenden....

 MEM ALPHA MODIFIKATION

BioWest

MEM Alpha ist eine Modifikation des Minimum Essential Medium (MEM), das nicht-essentielle Aminosäuren, Natriumpyruvat, Thioctsäure, Vitamin B12, Biotin und Ascorbinsäure enthält. MEM Alpha - Modifikation kann mit einer Vielzahl von Suspensions- und adhärenten Säugetierzellen verwendet werden, darunter Keratinozyten, primäre Rattenastrozyten und menschliche Melanomzellen....

Dulbecco's Phosphate Buffered Saline (DPBS) w/o Calcium w/o Magnesium. Sterile

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Wasser für die Zellkultur, Pyrogen frei

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Dulbecco's Phosphate Buffered Saline (DPBS) 10X w/o Calcium w/o Magnesium

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Dulbecco's Phosphate Buffered Saline (DPBS) w/o Calcium w/o Magnesium, Powder

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Trypsin-EDTA 10X in PBS w/o Calcium w/o Magnesium w/o Phenol Red

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X0930-100

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Hanks' Balanced Salts Solution (HBSS) w/ Calcium w/ Magnesium w/ Sodium Bicarbonate w/o Phenol Red

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L0612-500

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Potassium Chloride 0.075 M

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L0643-100

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Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/ Phenol Red

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Accutase

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Hanks' Balanced Salts Solution (HBSS) w/o Calcium w/o Magnesium w/o Sodium Bicarbonate w/o Phenol Red

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L0605-500

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MEM non Essential Amino Acids 100X w/o L-Glutamine

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Hanks' Balanced Salts Solution (HBSS) w/o Calcium w/o Magnesium w/ Sodium Bicarbonate w/ Phenol Red

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DULBECCO'S PHOSPHATE BUFFERED SALINE, M

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HEPES Buffer solution,1 M, steril

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Glutamine stable 100X, 200mM

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X0551-100

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HANKS' BALANCED SALT SOLUTION, MODIFIED, WITH SODIUM BICARBONATE, WITHOUT PHENOL RED, CALCIUM CHLORIDE AND MAGNESIUM SU

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Sodium Bicarbonate 7.5 %

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L0680-100

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Tween® 80 (Polysorbat) Ph Eur,NF,JP

Merck

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Tween® 80 zur Synthese

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Wasser, deionisiert, steril, pyrogenfrei, Zellkultur getestet

SERANA

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ACCUTASE® LÖSUNG

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RPMI-1640 MEDIUM, WITH SODIUM BICARBONATE, WITHOUT L-GLUTAMINE, STERILE-FILTERED. CELL CULTURE TESTED

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Trypsin 0.25% - EDTA in HBSS w/o Calcium w/o Magnesium w/ Phenol Red

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DULBECCO#S MODIFIED EAGLE#S MEDIUM - HIGH GLUCOSE, WITH 4500 MG/L GLUCOSE AND SODIUM BICARBONATE, WITHOUT L- GLUTAMINE A

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DMEM - F12 w/o L-Glutamine w/o HEPES w/o Glucose

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Dulbecco's PBS, ohne Calcium und Magnesium (DBPS), steril

SERANA

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Trypsin 0.25% - EDTA 0.02% in HBSS w/o Calcium w/o Magnesium w/ Phenol Red

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Colcemid 10
µ
g/ml in PBS (Demecolcin)

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L0040-020

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Sodium Pyruvate 100 mM

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DULBECCO#S MODIFIED EAGLE#S MEDIUM - HIGH GLUCOSE, WITH 4500 MG/L GLUCOSE, L-GLUTAMINE, AND SODIUM BICARBONATE, WITHOUT

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MEM NON-ESSENTIAL AMINO ACID (100X)*SOLU TION STERILE

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GHS05
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MINIMUM ESSENTIAL MEDIUM EAGLE W/L- *GLUT AMINE IN

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MINIMUM ESSENTIAL MEDIUM EAGLE, WITH HANKS' SALTS AND SODIUM BICARBONATE, WITHOUT L-GLUTAMINE, STERILE- FILTERED. CELL C

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Trypsin 0.25 % in PBS w/o Calcium w/o Magnesium w/o Phenol Red

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L0910-100

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DULBECCO#S PHOSPHATE BUFFERED SALINE, WITH MGCL2 AND CACL2, STERILEFILTERED. CELL CULTURE TESTED

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Dulbecco's PBS (10X), 10X DPBS without calcium and magnesium

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BDL-002-500ML

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Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium w/o Phenol Red

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DULBECCO#S MODIFIED EAGLE#S MEDIUM - LOW GLUCOSE, WITH 1000 MG/L GLUCOSE, L-GLUTAMINE, AND SODIUM BICARBONATE, STERILE-

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DULBECCO#S PHOSPHATE BUFFERED SALINE, 10X, MODIFIED, WITHOUT CALCIUM CHLORIDE AND MAGNESIUM CHLORIDE, STERILE-FILTERED.

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HANKS' BALANCED SALT SOLUTION, MODIFIED, WITH SODIUM BICARBONATE, WITHOUT CALCIUM CHLORIDE AND MAGNESIUM SULFATE. STERI

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Leibovitz L15 Medium w/o L-Glutamine

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L0300-500

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MEDIUM 199, WITH EARLE'S SALTS, LGLUTAMINE AND SODIUM BICARBONATE, STERILE-FILTERED. CELL CULTURE TESTED

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RPMI-1640 MEDIUM, WITH L-GLUTAMINE AND SODIUM BICARBONATE, STERILEFILTERED. CELL CULTURE TESTED

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Dulbecco's Phosphate Buffered Saline (DPBS) 10X w/ Calcium w/ Magnesium

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X0520-500

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MINIMUM ESSENTIAL MEDIUM EAGLE, WITH EARLE'S SALTS AND SODIUM BICARBONATE, WITHOUT L-GLUTAMINE. STERILEFILTERED. CELL

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RPMI 1640 w/o L-Glutamine

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RPMI 1640 w/o L-Glutamine w/o Phenol Red

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L0505-500

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100 mL, Cell Culture Grade Water, Tested to USP Sterile Water for Injection Specifications

Corning

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1

7647413

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AAT MEDIA SUPPLEMENT (50X) GAMMA- *IRRADI ATED CELL

Sigma-Aldrich

GHS06 GHS08
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A5539-1VL

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Accutase Cell Detachment Solution

SERANA

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1

RTL-007-100ML

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ADCF MAb

Cytiva

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SH30349.01

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500 ml Login / Register here
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ADCF MAb

Cytiva

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SH30349.02

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1 l Login / Register here
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ADCF MAb

Cytiva

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SH30349.LS

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6 l Login / Register here
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AdvanceSTEM Basal Medium for Undifferentiated Human Mesenchymal Stem Cells

Cytiva

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SH30879.01

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AdvanceSTEM Basal Medium for Undifferentiated Human Mesenchymal Stem Cells

Cytiva

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SH30879.02

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AdvanceSTEM DMEM4SC, with 4500 mg/L Glucose, without LGlutamine and Sodium Pyruvate

Cytiva

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SH30824.01

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AdvanceSTEM ES Qualified HEPES Solution (1M)

Cytiva

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SH30851.01

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100 ml Login / Register here
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AdvanceSTEM ES Qualified NonEssential Amino Acids Solution (100X)

Cytiva

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SH30853.01

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100 ml Login / Register here
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AGARGEL PLANT CELL CULTURE TESTED

Sigma-Aldrich

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A3301-500G

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A3301-1KG

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A3301-5KG

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Alpha Calf Fraction, without Iron

Cytiva

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SH30212.03

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AMES# MEDIUM, WITH L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTED

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A1420-10X1L

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10X1L Login / Register here
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Amphotericin B Solution

SERANA

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1

RAL-005-100ML

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ANTIBIOTIC ANTIMYCOTIC SOLUTION (100X), STABILIZED

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A5955-20ML

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A5955-100ML

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ANTIFOAM 204, ORGANIC, AUTOCLAVED

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A8311-50ML

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AT MEDIA SUPPLEMENT (50X) CELL CULTURE*T ESTED

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GHS06 GHS08
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A7422-1VL

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Bacillus Cereus- Selektivsupplement für die Zubereitung von 5 l Cereus Selektivagar MOSSEL

Merck

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1.09875.0010

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10FLASCHEN Login / Register here
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Balanced Salt Solution (BSS), sterile filtered, abgefüllt in 100ml-Flasche

SERANA

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BBL-002-25ML

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25 ml Login / Register here
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BANANA POWDER PLANT CELL CULTURE TESTED

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B4032-500G

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Basal Medium Eagle - BME w/ Earle's Salts w/ L-Glutamine w/o Sodium Bicarbonate

BioWest

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P0030-N1L

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P0030-N10L

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Basal Medium Eagle - BME w/ Earle's Salts w/o L-Glutamine w/ 25 mM HEPES

BioWest

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L0044-100

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L0044-500

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Basal Medium Eagle - BME w/ Hanks' Salts w/o L-Glutamine

BioWest

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L0046-100

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L0046-500

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BASAL MEDIUM EAGLE, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTED

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B9638-10L

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B9638-10X1L

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BASAL MEDIUM EAGLE, WITH EARLE'S SALTS AND NAHCO3, WITHOUT L- GLUTAMINE, STERILE-FILTERED, CELL CULTURE TESTED

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B1522-500ML

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BME 10X w/ Earle's Salts w/o L-Glutamine w/o Sodium Bicarbonate

BioWest

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X0042-100

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X0042-500

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BME 10X W/ Hanks' Salts w/o L-Gutamine w/o Sodium Bicarbonate

BioWest

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X0026-100

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X0026-500

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BME AMINO ACIDS (50X) SOLUTION*STERILE F ILTERED CELL

Sigma-Aldrich

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B6766-100ML

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CCM3

Cytiva

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SH30061.03

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286 g Login / Register here
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CCM3

Cytiva

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SH30065.02

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1 l Login / Register here
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CDM4CHO with LGlutamine

Cytiva

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SH30557.02

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1 l Login / Register here
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CDM4CHO without LGlutamine

Cytiva

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SH30556.01

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85.7 g Login / Register here
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CDM4CHO without LGlutamine

Cytiva

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SH30558.02

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1 l Login / Register here
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