DNA und RNA Isolierung

Hochreine DNA- und RNA-Proben können aus einer Vielzahl von verschiedenen Quellen extrahiert werden und sind extrem wichtig für zahlreiche Forschungs- und klinische Anwendungen. Die Isolierung von Nukleinsäure beinhaltet oftmals eine Lyse von Zellmembranen oder Probenhomogenisierung. Verschiedene hochwertige Aufreinigungs-Kits, Reagenzien und Hilfsmittel für die Aufreinigung sind von z.B. Macherey-Nagel, IST Innuscreen, Promega, NEB und weiteren Anbietern bei Th. Geyer erhältlich.

Filter

 Plasmidreinigungs-Kits I-Blue Mini / Midi

IBI Scientific

Für die schnelle und effiziente Extraktion von Plasmid DNA aus Bakterienkulturen.
Die Methode basiert auf einer Kombination aus alkalischer Lyse und RNase-Behandlung, um ein klares Lysat mit minimaler genomischer DNA- und RNA-Kontamination zu erhalten. Die gebundene Plasmid-DNA wird anschließend gewaschen und final durch die Zugabe eines Puffers eluiert. Im Testkit enthalten ist der I-Blue Lyse-Puffer, ein optionaler Farbindikator. Die Verwendung dieses Indikators vermeidet häufige Handhabungsfehler, die eine effiziente Zelllyse und Neutralisation beeinträchtigen....

 DNA-Leitern für Gelelektrophorese

Cleaver

Die gebrauchsfertigen DNA-Marker laufen präzise und erzeugen eine scharfe, gut definierbare Leiter. Sie sind in sechs Molekulargewichtsbereichen erhältlich. Die Marker enthalten hochintensive Referenzbanden und können für Größenvergleiche mit DNA-Molekülen von kleinsten PCR-Fragmenten bis hin zu großen, linearisierten Cosmid-Vektoren verwendet werden....

 Magnetische Racks für Trennverfahren mittels magnetischer Nanopartikel

Bel-Art

Geeignet für Affinitäts-basierte Trenn- und Reinigungsverfahren mittels magnetischer Nanopartikel z.B. Antikörper oder Nukleinsäure-Reinigungen.

  • Modelle für 1,5 / 2,0 ml, 5 / 15 ml bzw. 50 ml Röhrchen
  • Unabhängige und drehbare Röhrchenhalter mit leistungsstarken Magneten (zum Patent angemeldet) bei 50 ml und 1,5 / 5ml Modellen
  • Einfache Zugabe und Entfernung von Flüssigkeiten
  • 96-well PCR-Plattenmodell mit 24 Magneten
  • Allgemeines Modell für Mikroplatten mit mehreren Magneten geeignet für z.B. 6-, 12-, 24-, 48-, 96-Well Platten
  • Racks passend für Mikroplatten gemäß ANSI-Standards
  • Röhrchenhalter aus durchsichtigem klaren Acryl ermöglicht ungehinderten Blick auf den Röhrcheninhalt
...

 Mikroplatten Riplate® magnetic

Ritter

Die Riplate ® magnetic eignen sich speziell zur reproduzierbaren Reinigung von DNA und Proteinen mittels Magnetseparation sowie zum Mischen oder Zentrifugieren von Proben und zur Lagerung und Aufbereitung von Substanzen.
Zur Aufreinigung von Proteinen sowie zur DNA-Extraktion wird vielfach eine automatisierte Magnetseparation eingesetzt. Diese auf magnetischen Partikeln basierende Technologie ermöglicht eine schnelle und effiziente Probenvorbereitung mit hohem Durchsatz. Durch die Produktion unter Reinraumbedingungen der Klasse 8 garantiert Ritter die Nachhaltigkeit dieser Produkte sowie zuverlässige Ergebnisse der Analysen. Die Platten entsprechen dem SBS-Standard (Society for Biomolecular Screening) und sind speziell für Automaten der Magnetseparation entwickelt worden....

 Spin-Säulen, Glasfaserfilter

LLG

Die Spin-Säulen enthalten jeweils 2 Filter und sind speziell für Aufreinigungen von DNA (GF-F2) oder RNA (GF-N2) empfohlen. Sie bestehen aus 0,8 ml Filter-Röhrchen und 1,5 ml oder 2,0 ml Auffang-Röhrchen.

  • Empfohlen für die Aufreinigung
  • Vorfiltration von verunreinigten Lösungen
  • Hohe Durchflussleistung
  • Hohe Partikelbeladungskapazität
...

 NucleoSpin® 8 / 96 Gewebe

MACHEREY-NAGEL
GHS02

...

 Genomische DNA aus Blut

MACHEREY-NAGEL
GHS02

...

 NucleoMag™ Virus

MACHEREY-NAGEL
GHS02

Kit mit magnetischen Beads zur Isolierung viraler RNA und DNA aus zellfreien biologischen Flüssigkeiten. Geeignet für den automatisierten Einsatz....

 NucleoMag™ DNA/RNA Water

MACHEREY-NAGEL
GHS02

Kit zur DNA und RNA Isolierung aus Wasser- und Luftproben mittels magnetischer Beads. Geeignet für den automatisierten Einsatz....

 NucleoMag™ Pathogen

MACHEREY-NAGEL
GHS02

Kit mit magnetischen Beads zur Isolierung viraler Nukleinsäuren und bakterieller DNA aus klinischen Proben. Geeignet für den automatisierten Einsatz....

 Isolierung viraler RNA und DNA

MACHEREY-NAGEL
GHS02

...

 NucleoSpin™ Dx Virus

MACHEREY-NAGEL
GHS02

Das NucleoSpin™ Dx Virus Kit zur Extraktion von viraler Nukleinsäuren, aus menschlichen Serum oder Plasma, für nachfolgende in-vitro diagnostische Untersuchungen. Die isolierten und aufgereinigten Nukleinsäuren können in qualitativen Anwendungen (z.B. RT-PCR) als auch in quantitativen Anwendungen (z.B. Nachweis der Viruslast durch qPCR) verwendet werden....

 NucleoSpin™ RNA Plus

MACHEREY-NAGEL
GHS02

Mini Kit für RNA Aufreinigung mittels DNA Removal Säule. Ultraschnelle und praktische RNA Isolierung ohne rDNase Verdau....

 Nucleospin™ RNA Virus

MACHEREY-NAGEL
GHS02

Das Nucleospin™ RNA Virus Kit ist ein zuverlässiges und sicheres Verfahren, zur Isolierung von viraler RNA, frei von Verunreinigungen. Virale RNA innerhalb von nur 30 Minuten isolieren, dank des schnellen und praktischen Protokolls. Im Kit ist die Träger-RNA, für höchste Empfindlichkeit in Downstream-Prozessen, enthalten....

 NucleoSpin™ Virus

MACHEREY-NAGEL
GHS02

Mini spin kit zur zeitsparenden gleichzeitigen Isolierung viraler RNA und DNA....

Filterröhrchen, 0,8ml, klar ohne Filter, VE=1 Beutel 1000 Filterröhrchen / 1000 Filterringe

LLG

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6283187

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riplate® SRW 96 magnetic, 2 ml, PP VE=60

Ritter

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6272031

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NucleoBond Smart Rack light grey rack for use with NucleoBond Xtra Midi, Xtra Maxi, Xtra BAC, AX 20, AX 100, AX 500, AX 2000, and AX 10000 Columns pack of 1

MACHEREY-NAGEL

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740413

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NucleoSpin Plasmid (250) 250 preps for the isolation of plasmid DNA - NucleoSpin Plasmid Columns, Collection Tubes, buffers, RNase A + 81 Positionen Behälter

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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1

740588.250

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Collection Tubes (1000) Collection Tubes 2 mL, without lid pack of 1000

MACHEREY-NAGEL

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740600

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Tween® 20 (Polysorbat) Ph Eur,NF

Merck

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8.17072.1000

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8.17072.2500

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NucleoSpin Plasmid (50) 50 preps for the isolation of plasmid DNA - NucleoSpin Plasmid Columns, Collection Tubes, buffers, RNase A EQ Freigestellte Menge oder

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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1

740588.50

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Tween® 20 zur Synthese

Merck

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NucleoBond PC 2000 EF (5) 5 preps for the isolation of endotoxin-free plasmid DNA - NucleoBond AX 2000 Columns, NucleoBond Bottle Top Filters Type 1, buffers, RNase A

MACHEREY-NAGEL

GHS02
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740549

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NucleoBond Xtra Midi (100) 100 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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1

740410.100

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NucleoBond Xtra Midi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A

MACHEREY-NAGEL

GHS02 GHS07
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1

740410.50

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NucleoSpin Plasmid (NoLid) (250) 250 preps for the isolation of plasmid DNA - NucleoSpin Plasmid (NoLid) Columns, Collection Tubes, buffers, RNase A

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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1

740499.250

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NucleoSpin Plasmid Binding Strips (48) 8-well strips for the isolation of plasmid DNA

MACHEREY-NAGEL

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740730.48S

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NucleoSpin RNA (50) 50 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase EQ Freigestellte Menge oder

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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740955.50

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NucleoBond Xtra Maxi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

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GHS02 GHS07 GHS08
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1

740414.10

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NucleoBond Xtra Maxi (50) 50 preps for the isolation of plasmid DNA - NucleoBond Xtra Maxi Columns, NucleoBond Xtra Maxi Column Filters, buffers, RNase A

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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740414.50

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NucleoSEQ (250) pre-filled NucleoSEQ Columns, Collection Tubes pack of 250

MACHEREY-NAGEL

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1

740523.250

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NucleoSpin Plant II Columns (250) binding columns for the isolation of genomic DNA from plant samples pack of 250

MACHEREY-NAGEL

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1

740770.250S

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NucleoSpin Plasmid EasyPure (250) 250 preps for the isolation of plasmid DNA NucleoSpin Plasmid EasyPure Columns, Collection Tubes, buffers, Liquid RNase A

MACHEREY-NAGEL

GHS07
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1

740727.250

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NucleoSpin Plasmid QuickPure (250) 250 preps for the isolation of plasmid DNA - NucleoSpin Plasmid QuickPure Columns, Collection Tubes, buffers, RNase A

MACHEREY-NAGEL

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740615.250

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Product scheduled for discontinuation

NucleoSpin RNA (250) 250 preps for the isolation of total RNA - NucleoSpin RNA Columns, NucleoSpin Filters, Collection Tubes, buffers, RNase-free rDNase

MACHEREY-NAGEL

GHS02 GHS07 GHS08
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1

740955.250

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rDNase Set Recombinant DNase and rDNase Reaction Buffer, For RNA minipreparations with NucleoSpin kits

MACHEREY-NAGEL

GHS08
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1

740963

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1 Set Login / Register here
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0.5M TCEP

Sigma-Aldrich

GHS05
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646547-10X1ML

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1,4 Dithiothreitol (DTT) ANWENDUNG: Reducing agent WISSENSCHAFTSGEBIET: Biochemicals SONSTIGE INFOS: Cleland´s reagent. Protective reagent for sulfhydryl groups of proteins and enzymes. maintains monothiols in the reduced state and reduces disulfides quantitatively. Show more

Biomol

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04010.5

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Product scheduled for discontinuation

1,4-DITHIO-DL-THREIT-LSG., F. D. MOL.BIOL., ~1 M IN H2O*

Sigma-Aldrich

GHS07
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43816-10ML

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1,4-Dithiothreit für biochemische Zwecke

Merck

GHS07
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Adapter für 4 Reagent Strips, für InnuPure C16 und C16 touch

Analytik Jena

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7664646

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AGILENT RNA6000 NANO REAGENZ. ENTHAELT REAGENZIEN, LEITER UND MARKER

Agilent

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7626252

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BamH I restriction enzyme, 10.000 units, G | GATC C, C CTAG | G

TaKaRa

GHS07
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1010A

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Barcodereader (2D)

Analytik Jena

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7664642

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blackPREP Tick DNA/RNA Kit - 10 Reaktionen

IST Innuscreen

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845-BP-5100010

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blackPREP Tick DNA/RNA Kit - 50 Reaktionen

IST Innuscreen

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845-BP-5100050

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Buffer A1 (1000 mL) Resuspension Buffer A1, without RNase A

MACHEREY-NAGEL

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740911.1

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Buffer A1 (3000 mL) Resuspension Buffer A1, without RNase A

MACHEREY-NAGEL

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740911.3

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Buffer A1 (75 mL) Resuspension Buffer A1, without RNase A bottle of 75 mL

MACHEREY-NAGEL

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740911.75

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Buffer A2 (1000 mL) Lysis Buffer A2 without LyseControl

MACHEREY-NAGEL

GHS07
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1

740912.1

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Buffer A2 (3000 mL) Lysis Buffer A2 without LyseControl

MACHEREY-NAGEL

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740912.3

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Buffer A3 (1000 mL) Neutralization Buffer A3

MACHEREY-NAGEL

GHS07
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1

740913.1

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Buffer A3 (3000 mL) Neutralization Buffer A3

MACHEREY-NAGEL

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Buffer A4 Concentrate (200 mL) Wash Buffer A4 Concentrate, for 1000 mL Buffer A4 bottle of 200 mL

MACHEREY-NAGEL

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740914.1

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Buffer A4 Concentrate (25 mL) Wash Buffer A4 Concentrate, for 125 mL Buffer A4 bottle of 25 mL

MACHEREY-NAGEL

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740914

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Buffer A4 Concentrate (600 mL) Wash Buffer A4 Concentrate, for 3000 mL Buffer A4 bottle of 600 mL

MACHEREY-NAGEL

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740914.3

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Buffer AE (1000 mL) Elution Buffer AE

MACHEREY-NAGEL

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740917.1

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Buffer AE (3000 mL) Elution Buffer AE

MACHEREY-NAGEL

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740917.3

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Buffer AQ Concentrate (25 mL) Bottle of 25 mL Wash Buffer AQ Concentrate, for 125 mL Buffer AQ

MACHEREY-NAGEL

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740995

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Buffer AW (1000 mL) Wash Buffer AW

MACHEREY-NAGEL

GHS02
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1

740916.1

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Buffer AW (3000 mL) Wash Buffer AW

MACHEREY-NAGEL

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740916.3

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Buffer B3 (100 mL) Lysis Buffer B3 bottle of 100 mL

MACHEREY-NAGEL

GHS07
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1

740920

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Buffer B5 (100 mL) Wash Buffer B5 Concentrate, for 500 mL Buffer B5 bottle of 100 mL

MACHEREY-NAGEL

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740921.100

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Buffer B5 Concentrate (25 mL) Wash Buffer B5 Concentrate, for 125 mL Wash Buffer B5 bottle of 25 mL

MACHEREY-NAGEL

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740921

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Buffer BB (110 mL) Binding Buffer BB bottle of 110 mL

MACHEREY-NAGEL

GHS02
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1

740394.110

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Buffer BB (22 mL) Binding Buffer BB bottle of 22 mL EQ Freigestellte Menge oder

MACHEREY-NAGEL

GHS02
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1

740394.22

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Buffer BE (125 mL) Elution Buffer BE

MACHEREY-NAGEL

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740306.100

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Buffer BQ1 (125 mL) Lysis Buffer BQ1

MACHEREY-NAGEL

GHS07
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1

740923

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Buffer BQ2 Concentrate (50 mL) Wash Buffer BQ2 Concentrate bottle of 50 mL

MACHEREY-NAGEL

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1

740307.50

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Buffer BW (100 mL) Wash Buffer BW bottle of 100 mL

MACHEREY-NAGEL

GHS02
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1

740922

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Buffer BW (500 mL) Wash Buffer BW

MACHEREY-NAGEL

GHS02
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1

740922.500

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Buffer C1 (60 mL) Lysis Buffer C1 bottle of 60 mL

MACHEREY-NAGEL

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1

740930

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Buffer C4 (250 mL) Binding Buffer C4 bottle of 250 mL

MACHEREY-NAGEL

GHS07
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1

740366.250

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Buffer C5 Concentrate (25 mL) Wash Buffer C5 Concentrate, for 125 mL Buffer C5 bottle of 25 mL

MACHEREY-NAGEL

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1

740931

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Buffer CB (100 mL) Lysis Buffer CB bottle of 100 mL

MACHEREY-NAGEL

GHS02
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1

740327

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Buffer CF (1000 mL) Lysis Buffer CF

MACHEREY-NAGEL

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1

740946

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Buffer CQW (125 mL) Wash Buffer CQW

MACHEREY-NAGEL

GHS02
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1

740313.125

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Buffer CW (100 mL) Wash Buffer CW bottle of 100 mL

MACHEREY-NAGEL

GHS02
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1

740932

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Buffer DB (1000 mL) Binding Buffer DB

MACHEREY-NAGEL

GHS02
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1

740323.1000

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Buffer DB (25 mL) Binding Buffer DB bottle of 25 mL EQ Freigestellte Menge oder

MACHEREY-NAGEL

GHS02
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1

740323.25

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Buffer DL (100 mL) Lysis Buffer DL bottle of 100 mL

MACHEREY-NAGEL

GHS07
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1

740202.32

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Buffer ELU (600 mL) Elution Buffer ELU bottle of 600 mL

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740316.600

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Buffer ELU (900 mL) Elution Buffer ELU bottle of 900 mL

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GHS02
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740316.900

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Buffer ELU-EF (3000 mL) Elution Buffer ELU-EF

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GHS02
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740393.3000

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Buffer ELU-EF (900 mL) Elution Buffer ELU-EF bottle of 900 mL

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740393.900

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Buffer ENDO-EF (1000 mL) Wash Buffer ENDO-EF

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GHS02
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740391.1000

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Buffer ENDO-EF (3000 mL) Wash Buffer ENDO-EF

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GHS02
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740391.3000

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Buffer EQU (1000 mL) Equilibration Buffer EQU

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740317.1000

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Buffer EQU-EF (1000 mL) Equilibration Buffer EQU-EF

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740389.1000

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Buffer EQU-EF (3000 mL) Equilibration Buffer EQU-EF

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GHS02
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740389.3000

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Buffer FIL-EF (3000 mL) Wash Buffer FIL-EF

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740390.3000

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Buffer FIL-EF (600 mL) Filter Wash Buffer FIL-EF bottle of 600 mL

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740390.600

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Buffer FLB (500 mL) Lysis Buffer FLB

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740322.500

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Buffer LB1 (30 mL) Lysis Buffer LB1 bottle of 30 mL

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740368.30

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Buffer LB2 (30 mL) Lysis Buffer LB2 bottle of 30 mL

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740369.30

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Buffer LBP (125 mL) Lysis Buffer LBP

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GHS07
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740906.125

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Buffer LYS (1000 mL) Lysis Buffer LYS

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GHS07
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740329.1000

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Buffer LYS-EF (1000 mL) Lysis Buffer LYS

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GHS07
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740387.1000

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Buffer LYS-EF (3000 mL) Lysis Buffer LYS-EF

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GHS07
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1

740387.3000

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