Cell culture media and reagents

For cell culture, Greiner Bio-One offers standard and filter cap cell culture flasks with growth areas of four different sizes. Suspension culture flasks complete the range in another sizes.They are made of high-grade polystyrene. The special design of our cell culture flasks makes it possible to efficiently reach the cell lawn with a cell scraper, whilst still providing optimal access with a pipette. The special neck
design reduces the risk of wetting the inside of the cap with medium, thus providing additional protection against contamination. The stacking rim on the top of the flasks ensures firm standing and easy stackability in the incubator.

Properties:

• Improved cell adhesion through physical surface treatment
• Cell culture flasks with standard screw cap (without filter)
• Canted neck
• Graduation on both sides
• Sterile and user-friendly packaging
• 25 cm², 75 cm² and 175 cm² growth areas
• High and flat design of the 175 cm² cell culture flask for variability of media volume

The DMEM medium is a modification of the BME which contains a higher concentration of amino acids, vitamins and other additives. DMEM is for supporting and maintaining a vast range of mammalian cell types. There are two types of DMEM: one with a high glucose content (4.5g/L), and the other with a low glucose content (1.0g/L). The DMEM low glucose has been developed for the culture of mouse embryonic cells.

Minimum Essential Medium (MEM) with Earle’s Balanced Salts is a modification of Eagle’s earlier Basal Medium (BME) which contains a higher concentration of essential nutrients. These media promote the growth of a variety of normal and transformed cells. Since they contain Earle’s Balanced Salts, they are suitable for use in atmospheres charged with CO2 gas.

RPMI are general purpose enriched media with extensive applications for a broad spectrum of mammalian and hybridoma cells, including human myeloma, mouse hybridoma, human leukocytes, and B and T lymphocytes. It was originally formulated for suspension cultures and monolayer culture of human leukemia cells.

The DMEM Ham’s F12 offers excellent performance for certain epithelial, endothelial and granulose cell types. With proper supplementation, it is a highly successful basic media for serum free cell culture.

Ham's F12 was originally developed for serum-free clonal growth of Chinese hamster ovary (CHO) cells, lung cells and mouse L-cells. It is the medium of choice for supporting cells from rodents (especially rabbit and rat) and has proven to be an excellent
cloning medium for the culture of myeloma and hybrid cells (hybridomas).

Minimum Essential Medium (MEM) is a modification of Eagle’s earlier medium Basal Medium Eagle (BME). This MEM w/ Hanks’ Salts formulation contains Hank’s salts for use without CO2.

Protect your cell lines from contamination by foreign organisms such as bacteria, fungi and viruses and/or use the antibiotics for selection after transfection.
Most antibiotics suppress the growth of micro-organisms by blocking an anabolic pathway.
Nanomycopulitine actively kills bacteria, including mycoplasma, in all three stages of development without interfering with the eukaryotic metabolism.

BME ist der Vorgänger von Eagle Minimum Essential Medium (MEM) und Dulbecco Modified Eagle Medium (D-MEM). Es ist das einfachste der Basismedien mit allen wesentlichen Komponenten für das Zellwachstum. Basalmedium Eagle (BME) begünstigt idealerweise Zelllinien wie HeLa, L-Zellen und primäre Säugetierfibroblasten.

Iscoves media are enriched modifications of DMEM containing sodium selenite. They are excellent for rapidly proliferating high-density cell cultures. The addition of BSA, purified human transferring and soybean lecithin creates a serum free condition ideal for supporting B and T lymphocytes. IMDM was the first media utilizing HEPES buffer. Other cell types can be cultured using this medium under serum free or reduced serum conditions.

MEM á is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, thioctic acid, vitamin B12, biotin, and ascorbic acid. MEM á - Modification can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells.

Mc Coy’s media were originally formulated for growth and support of lymphocytes. This final modification produced a medium identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells. In addition, it is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal or transformed cell types, the most current primary and continuous cell lines.

This complex medium was developed specifically for nutritional research of chicken
fibroblasts. Today, 199 media are widely used for the maintenance of non-transformed cells, vaccine and virus production and primary explants of epithelial cells. The media can be formulated either with Earle salts or Hanks Salts. The media formulated with the Earle salts are buffered with a bicarbonate / carbonic acid solution and retain their pH in a CO2 incubator. The use of the Earle salts under ambient conditions results in a rapid rise in the pH of the culture medium.
The media formulated with the Hanks salts are buffered with saline solutions designed for balancing in ambient conditions, and their use in a CO2 incubator results in a rapid drop in the pH of the culture medium.

CMRL-Medien sind besonders für das klonen von Affen Nierenzellen und für das Wachstum vieler anderer Säugetierzelllinien geeignet, wenn sie mit Pferde- oder Kälberserum ergänzt werden.

Glascow Minimum Essential Medium was originally developed by Ian MacPherson and Michael Stoker as a modification of Eagle’s medium (BME). The modifications included adding 10% tryptose phosphate and twice the normal concentration of amino acids and vitamins. This medium was used to study the genetic factors affecting cell competence.

Ham's F10 ist ein beliebtes Medium für das Wachstum von anspruchsvollen Zelllinien.

Ham’s F14 medium was developed from the Ham’s F12, which was originally developed for the serum-free clonal growth of Chinese Hamster Ovary (CHO) cells, lung cells and mouse L-cells. Ham’s F14 contains a double concentration of amino acids compared to the Ham’s F12. This product is also supplemented with Calcium Chloride and Ascorbic Acid.

The Leibovitz L-15 media were formulated to promote the cell growth in
medium not balanced in CO2. The formulations were developed with the sodium
bicarbonate buffer. The Leibovitz L-15 media are buffered by a complement of salts,
free base amino acids and galactose, so they can be used under conditions of free
gaseous exchange with the atmosphere. When properly supplemented, L-15 Medium supports established cell lines, such as HEp-2 and LLC-MK2, as well as primary explants of embryonic and adult human.

These media were formulated as a reduced serum supplemented medium for the cultivation of human microvascular endothelials cells. For other microvascular cells, it is recommended to be used with growth factors, like EGF or hormones like hydorcortisone.