Elektroporation

BTX Cuvettes Plus are designed for use in electroporation and electrofusion of bacteria, yeast, insect, plant and mammalian cells. Each sterilized Cuvettes Plus package includes a disposable cuvette and a transfer pipette, which is used for removal of the sample after electroporation. The cuvettes are molded with embedded polished aluminum electrodes, and gamma irradiated for guaranteed sterility.
Three electrode gap sizes are available:

• 1mm for bacteria and yeast
• 2mm for all cell types
• 4mm for mammalian cells

BTX cuvette Plus can obtain high field strengths up to 25.0 kV/cm. Round caps are leak resistant and allow for quick and easy removal. For research use only. Can be used with electroporation systems from different manufacturers (not suitable for units from Eppendorf).

The ECM^® 399 is an electroporation system for the simple transformation of bacteria and yeast cells. It produces exponential decay waves and is designed to deliver the field strengths and pulse lengths required for transformations. The ECM^® 399 is ideal for basic transformation in research and academic environments. It is easy to operate, cost effective, compact in size and portable.

Applications: Transformation of bacteria, yeast, fungus.

Scope of supply: Generator ECM^® 399 , PEP cuvette holder, Cuvettes 1 mm, 2 mm, 4 mm (10 each) and Cuvette Rack

Specifications
Capacity/Resistance Range:
LV Mode: HV Mode:	1050 µF / 150 Ohm, fixed 36 µF / 150 Ohm, fixed
Max. Voltage Output:	2500 V (Peak)
Pulse Interval: Charge Time:	100 ms to 10 s max. 5 s
Max. Pulse Length:	125 ms at 500 V (Peak) or 5 ms at 2500 V (Peak)
Display:	16-character, liquid crystal, LCD backlit
Interface: Relative Humidity:	Digital User Interface 60 %
Power supply:	100 - 240 V, 50 / 60 Hz

Accessories Electroporation cuvettes Plus

The versatile electroporation system is suitable for bacterial and yeast cell transformations, as well as for transfections of mammalian cells and tissues. The supplied safety dome connects to the generator to safely deliver high-voltage electrical pulses to cuvettes. Up to two cuvettes can be electroporated simultaneously.

• Large, easy-to-use touchscreen interface
• Preset protocols for commonly used mammalian cell lines and tissues
• Storage of up to 1000 user-defined protocols
• Safety functions: Pre-pulse resistance check, arc protection, and over-current pulse abort
• Protocol of each delivered pulse for quality control and troubleshooting

Scope of supply: Generator ECM^® 830, safety dome, electroporation cuvettes 1 mm / 2 mm / 4 mm (10 each), cuvette rack

Capacitance LV mode:	3775 µF
Capacitance HV mode:	85 µF
Voltage LV mode:	5 ... 500 V
Voltage HV mode:	505 ... 3000 V
Multiple pulse:	1 ... 99 pulses per sample
Pulse length LV mode:	10 µs ... 999 ms
Pulse length HV mode:	10 µs ... 600 µs
Pulse interval:	100 ms ... 10 s
Charging time LV mode:	<7 s
Charging time HV mode:	<4 s

Accessories Electroporation cuvettes Plus